Near-total glutathione depletion and age-specific cataracts (白內障) induced by buthionine sulfoximine in mice [1986](IR91)
http://www.ncbi.nlm.nih.gov/pubmed/3726547
Science 1 August 1986:
Vol. 233 no. 4763 pp. 553-555
DOI: 10.1126/science.3726547
Near-total glutathione depletion and age-specific cataracts (
白內障) induced by buthionine sulfoximine in mice
HI Calvin, C Medvedovsky and BV Worgul
ABSTRACT
The specific inhibitor of glutathione biosynthesis, L-buthionine sulfoximine (L-BSO), although relatively nontoxic in adult mice, induces severe glutathione depletion and age-specific pathological changes when repeatedly administered to male suckling mice. Dense (濃密的) cataracts (白內障) developed when mice aged 9 to 12 days were given a series of injections of L-BSO, despite excellent survival and the absence of other significant long-term effects. By contrast, similar treatment of mice aged 14 to 17 days, although slightly less effective in reducing glutathione levels, resulted frequently in death, hind-leg paralysis, or impaired spermatogenesis, but did not produce cataracts. Administration of L-BSO to preweanling mice provides a novel model system for the induction of cataracts (白內障) by depletion of lens glutathione and may enable the study of critical functions of glutathione in the lens and other growing tissues during early postnatal (產後的;出生後的) development.
http://www.ncbi.nlm.nih.gov/pubmed/3726547
Science 1 August 1986:
Vol. 233 no. 4763 pp. 553-555
DOI: 10.1126/science.3726547
Near-total glutathione depletion and age-specific cataracts (
白內障) induced by buthionine sulfoximine in mice
HI Calvin, C Medvedovsky and BV Worgul
ABSTRACT
The specific inhibitor of glutathione biosynthesis, L-buthionine sulfoximine (L-BSO), although relatively nontoxic in adult mice, induces severe glutathione depletion and age-specific pathological changes when repeatedly administered to male suckling mice. Dense (濃密的) cataracts (白內障) developed when mice aged 9 to 12 days were given a series of injections of L-BSO, despite excellent survival and the absence of other significant long-term effects. By contrast, similar treatment of mice aged 14 to 17 days, although slightly less effective in reducing glutathione levels, resulted frequently in death, hind-leg paralysis, or impaired spermatogenesis, but did not produce cataracts. Administration of L-BSO to preweanling mice provides a novel model system for the induction of cataracts (白內障) by depletion of lens glutathione and may enable the study of critical functions of glutathione in the lens and other growing tissues during early postnatal (產後的;出生後的) development.
