Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94)
在台灣地區的華人男性和女性志願者的血漿中之 [穀胱甘肽 （glutathione）] 的濃度（水平），隨著年齡的增長而逐漸地下降。
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(Memo Item created on February 20, 2009 02:33 PM)
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Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94)- - - End title or keyword:
Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection.
CS Yang, ST Chou, L Liu, PJ Tsai, and JS Kuo
J Chromatogr B Biomed Appl, December 1, 1995; 674(1): 23-30.
A convenient method for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) in human plasma by high-performance liquid chromatography with fluorescence detection is reported. This assay involves direct addition of human plasma to methanolic monobromobimane, for simultaneous protein precipitation and thiol derivatization. The assay was validated by addition of authentic GSH and GSSG to plasma samples. Plasma glutathione levels in Chinese male and female volunteers were found to decrease with increasing age (age groups 20-30, 30-40, 40-50, 50-60, and > 60; mean +/- S.E.M. 0.95 +/- 0.03, 0.77 +/- 0.02, 0.67 +/- 0.03, 0.51 +/- 0.02, 0.48 +/- 0.02 microM for male volunteers and 1.11 +/- 0.06, 0.76 +/- 0.03, 0.61 +/- 0.03, 0.53 +/- 0.04 and 0.43 +/- 0.04 microM for female volunteers). GSSG levels, in both males and females, did not show a correlation with age. There were no significant differences in GSH or GSSG levels among male and female volunteers of the same age group. These results suggest that elderly persons might be more susceptible to oxidative injury due to decreased plasma glutathione levels.
Publication Types:
Journal article
PMID: 8749248
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English Keywords:
Glutathione, cysteine, decrease, aging, old, less, deficiency, lower, not enough, hospital, near, center of, Taiwan
Glutathione, cysteine, decrease, aging, old, less, deficiency, lower, not enough, hospital, near, center of, Taiwan
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學術論文的圖檔_在台灣地區的華人男性和女性志願者的血漿中之 [穀胱甘肽 （glutathione）] 的濃度（水平），隨著年齡的增長而逐漸地下降。 [1995]_Cover.jpg
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學術論文的圖檔_在台灣地區的華人男性和女性志願者的血漿中之 [穀胱甘肽 （glutathione）] 的濃度（水平），隨著年齡的增長而逐漸地下降。 [1995]_Abstract.jpg
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